Journal: Cells

Article Title: Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures

doi: 10.3390/cells13030258

Figure Lengend Snippet: List of primary antibodies used in the present study.

Article Snippet: rabbit monoclonal anti-osteopontin , 1:1000 , Boster Biological Technology, Pleasanton, CA, USA.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Collagen-induced arthritis (CIA) enhanced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) expression in paw joint cartilage, while the Bi-Qi capsule or methotrexate (MTX) treatment reduced this effect. Representative immunohistochemistry images of paw joint synovium with OPN and COMP immunostaining

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Immunostaining

Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Bi-Qi capsule (BQ) or methotrexate (MTX) reduced collagen-induced arthritis (CIA)-induced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) upregulation in serum, and mRNA and protein levels in paw joint tissue. a, b OPN and COMP levels in serum. c, d OPN and COMP mRNA expression in paw joint cartilage. e, f OPN and COMP protein quantification from immunohistochemistry images of paw joint synovium. Data are presented as mean ± SD from eight rats in each group. Significant effect of treatment compared to control: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 . Significant effect of treatment compared to arthritic group: #### P < 0.0001 . Significant effect of treatment compared to high-dose BQ (BQ-HD): $$ P < 0.01, $$$$ P < 0.0001 . Significant effect of treatment compared to MTX-group: & P < 0.05, &&&& P < 0.0001. BQ-MD, moderate-dose BQ

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Control

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: ( a ): Baseline clinical and pathological characteristics of bladder cancer patients. ( b ): Median and IQR of patients’ age and follow-up duration. ( c ): Expression intensity of SPP1.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Staining

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Expression pattern of SPP1 in pan-cancer. ( A ) The expression of the SPP1 mRNA level in multiple TCGA cancers and matching normal tissues. p < 0.001, except Thym cancer ( p = 0.7) and KICH cancer ( p = 0.53). ( B ) Increased mRNA expression level of SPP1 in bladder cancer. ( C ) Promoter methylation status of SPP1 in bladder cancer and matching normal tissues. All data were analyzed using the UALCAN web tool.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Methylation

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Molecular alterations of SPP1 in cancers. ( A ) High amplifications/mutations of the SPP1 gene in bladder cancer compared other cancer types. ( B ) Positions and mutation frequency in SPP1 in bladder cancer.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Mutagenesis

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Cytoplasmic expression of SPP1 in bladder carcinoma. Immunohistochemical staining of the bladder cancer tissue microarray using an SPP1 antibody. Figures showing: no expression ( A , B ), weak ( C , D ), moderate ( E , F ) and strong expression ( G , H ) of SPP1. Images were taken using 10× and 40× magnification objectives (scale bar equals 1 mm).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Nuclear SPP1 expression in bladder carcinoma. Immunohistochemical staining of the bladder cancer tissue microarray using an SPP1 antibody. Figures showing: no expression ( C , D ), and strong expression of SPP1 ( A , B ). Images were taken with 10× and 40× magnification objectives (scale bar equals 1 mm).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Correlation between cytoplasmic SPP1 expression and patients’ clinicopathological characteristics.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: SPP1 expression and patients’ survival. Kaplan–Meier survival curve for bladder cancer patients expressing cytoplasmic ( A ) and nuclear ( B ) patterns of SPP1 (low expression vs. high expression). Low SPP1 immunostaining is associated with poor overall survival (log-rank p = 0.022).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunostaining

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Enrichment analysis of SPP1 in bladder cancer. ( A ) Identification of SPP1-interacting genes. Protein–protein interaction map and hub genes of SPP1. The size of the hub is proportional to the expression level. ( B ) Identification of the SPP1 co-expression network. The figure was generated using the online cBioPortal database. ( C ) KEGG functional enrichment analysis of SPP1. The figure was generated using the online cBioPortal database.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Generated, Functional Assay

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Correlation between immune cells and SPP1 expression. TIMER analysis of the correlation between SPP1 expression and immune cells’ infiltration. Purity-adjusted Spearman’s rho across various cell types by different algorithms.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Relationship between SPP1 expression and immune checkpoint genes in bladder cancer. ( A ) Correlation analysis between SPP1 expression and immune checkpoint genes. ( B ) The expression of immune checkpoint genes in relation to SPP1 expression. Data were analyzed using the cBioPortal cancer genomics website on TCGA data. The p -value significance codes: *** ≤0.001, ** ≤0.01, * ≤0.05.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Biomolecules

Article Title: Pro-Calcifying Role of Enzymatically Modified LDL (eLDL) in Aortic Valve Sclerosis via Induction of IL-6 and IL-33

doi: 10.3390/biom13071091

Figure Lengend Snippet: The primary antibodies used for immunohistochemistry and Western blot analysis.

Article Snippet: SPP1 , Osteopontin (SPP1) antibody , IHC-P , 1:500 , Mouse , Antikörper-online (abbexa).

Techniques: Immunohistochemistry, Western Blot

Journal: Biomolecules

Article Title: Pro-Calcifying Role of Enzymatically Modified LDL (eLDL) in Aortic Valve Sclerosis via Induction of IL-6 and IL-33

doi: 10.3390/biom13071091

Figure Lengend Snippet: List of TaqMan gene expression assays.

Article Snippet: SPP1 , Osteopontin (SPP1) antibody , IHC-P , 1:500 , Mouse , Antikörper-online (abbexa).

Techniques: Gene Expression, Gene Assay

Journal: Biomolecules

Article Title: Pro-Calcifying Role of Enzymatically Modified LDL (eLDL) in Aortic Valve Sclerosis via Induction of IL-6 and IL-33

doi: 10.3390/biom13071091

Figure Lengend Snippet: eLDL enhances phosphate-induced calcification in cultured human VICs/myofibroblasts. ( A ) Representative example of confluent monolayers of human VICs/myofibroblasts treated with 1 mM phosphate containing pro-calcifying medium (PM) with (wells 11 & 33 containing 2.5 µg/mL eLDL, wells 12 & 34 containing 5 µg/mL eLDL) or without eLDL (wells 10 & 32) as indicated. Cells cultured in PM containing 1 mM inorganic phosphate (P i ) served as controls. Cells were fixed and calcium phosphate deposits were stained with alizarin red pH 4.4. ( B ) For quantification of the alizarin red staining, the alizarin–Ca 2+ complexes were extracted by addition of CPC. The amount of released dye was measured by spectrophotometry at 570 nm. ( C ) qPCR analysis of osteogenic gene mRNA in cultured VICs/myofibroblasts incubated for 7 days in PM containing 1 mM inorganic phosphate (P i ) in the presence (5 µg/mL) or absence of eLDL (control). The mRNA expression levels were normalized to GAPDH according to the 2 −ddCT method. Results from 12 independent experiments are shown. Bar values are means ± SD. ( D ) eLDL induced ANGPTL4 gene expression in VICs/myofibroblasts. ANGPTL4 gene expression was determined for cells exposed to eLDL (5 μg/mL) in combination with PM 1 mM P i for 7 days. Untreated cells (PM with 1 mM P i ) served as control. Results are presented as means ± SD, n = 12, *** p < 0.001, ** p < 0.01, and * p < 0.05. ( E ) Immunohistochemical analysis of osteogenic proteins in AS. Representative sections of Grade 4 aortic valve calcification for ( a ), ENPP1, and ( b ), SPP1. Note the predominant localization of the different antigens around calcified areas. In all panels, the fibrosa with the aortic side of the valve is to the top.

Article Snippet: SPP1 , Osteopontin (SPP1) antibody , IHC-P , 1:500 , Mouse , Antikörper-online (abbexa).

Techniques: Cell Culture, Staining, Spectrophotometry, Incubation, Control, Expressing, Gene Expression, Immunohistochemical staining